Cortisone CLIA Kit

Cortisone CLIA Kit

Cortisone CLIA Kit

id: SKT-206-96


Cortisone (C21H28O5, Kendall's Compound ?E') was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin (1). Compound E is now called cortisone and the more active Compound F, cortisol, and the concentrations of these two glucocorticoids vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD) (2,3). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid ?shuttle? helps to initiate and regulate the anti-inflammatory response, making cortisone one of the modern ?wonder drugs?. onitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases (4-7). Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. A recent publication (8) has suggested that salivary cortisone is a good surrogate marker for serum cortisol.

Sandwich CLIA kit used for quantitative measuring the cortisone present in Dried Fecal Samples, Serum, Saliva, Urine, Plasma, Tissue Culture Media samples from all species

Assay type: Sandwich CLIA (Chemiluminescent Immunoassay)
Species reactivity: Species Independent
Sample types: Dried Fecal Samples, Serum, Saliva, Urine, Plasma, Tissue Culture Media
Sensitivity: 10.6 pg/ml
Assay range: 78.1 - 20,000 pg/ml
Incubation t 2 Hours
Number of samples per kit: 37 samples in duplicate
Method of detection: Chemiluminescent Assay

The kit is transported on ice packs (+4ºC). Upon receipt store at 4ºC. Non-hazardous item according to both ADR and UN regulations.

For research use only. Not to be used for human or animal treatment or diagnostics.


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